@pein
here is one of the many experiments on evolution of DNA from RNA being conducted..
for your own purposes..
click on this link -->
ScienceDirect - Biophysical Chemistry : Machines for automated evolution experiments in vitro based on the serial-transfer concept
another is this
http://www.dna.caltech.edu/courses/c...891%296093.pdf
or read the abstract below
~~~
Copyright © 1997 Published by Elsevier Science B.V.
Machines for automated evolution experiments
in vitro based on the serial-transfer concept
Günther Strunk and Tobias Ederhof
Max-Planck-Institute für biophysikalische Chemie, Evolutionsgruppe, Am Fassberg 11, D-37077, Göttingen, Germany
Received 24 April 1997;
accepted 24 April 1997. ;
Available online 7 April 1998.
Abstract
Two machine setups for automated evolution experiments
in vitro are described. Both machines enable the monitoring of growing populations of RNA or DNA molecules in real time using high-sensitivity glass fiber laser fluorimeters and an automated sample handling facility for volumes in the microliter range. Growth conditions are kept constant by means of the serial-transfer technique, that is, the successive transfer of a small fraction of a growing population into a fresh solution containing no individuals prior to the transfer. The serial transfer technique was modified to work with large populations and constant growth conditions. In the single-channel evolution machine isothermal amplification reactions (
Qβ-system, 3SR, NASBA, SDA) are monitored successively in single test tubes. This machine is particularly well suited for the investigation of optimal adaptation to altered environmental conditions, as is experimentally demonstrated in the evolution of an RNA quasispecies using ribonuclease A as the selection pressure. The new variant of RNA appeared very rapidly (within approximately 80 generations) without stable intermediates, and it was selected by steadily increasing the RNaseA concentration during the serial-transfer experiment.
The other machine, which is described in the second part of this article, is a consequent extension of the single-channel machine, and was designed to allow the multichannel detection of up to 960 samples simultaneously. Thus, high-throughput screening can be applied to evolution experiments. In addition to monitoring isothermal amplification reactions, it is also possible to follow PCR amplifications through thin plastic foils. Initial experiments have demonstrated the suitability of the apparatus for uniformly processing samples and for performing thermocycling.
Author Keywords: serial-transfer experiment; Qβ-replicase; self-replicating systems; on-line detection; selection pressure; RNase A
References
[1]. International Workshop (April 18 to 20, 1991) Selection —Natural And Unnatural— In Biotechnology. At the Max-Planck-Institute for Biophysical Chemistry, Am Fassberg, W-3400 Göttingen, FRG.
[2]. S. Spiegelman, I. Haruna, I.B. Holland, G. Beaudreau and D. Mills. In: (4th ed.),
Proc. Natl. Acad. Sc USA 54 (1965), p. 919.
Full Text via CrossRef |
View Record in Scopus |
Cited By in Scopus (15)
[3]. D.R. Mills, R.E. Peterson and S. Spiegelman. In: (4th ed.),
Proc. Natl. Acad. Sc. USA 58 (1967), p. 217.
Full Text via CrossRef |
View Record in Scopus |
Cited By in Scopus (100)
.....
....